El módulo de Prácticas Externas corresponde a un trabajo de laboratorio eminentemente práctico encaminado a consolidar, perfeccionar y completar la formación adquirida a través de los diferentes módulos teóricos del máster. Esta estancia proporciona a los estudiantes la oportunidad de ganar experiencia y aprender a desenvolverse en el entorno profesional.

Las estancias de prácticas duran entre tres y seis meses y se pueden realizar en un laboratorio de investigación del VHIR o en una institución externa, como centros de investigación o empresas farmacéuticas o biotecnológicas. Durante este periodo, el estudiante estará supervisado por un tutor de prácticas, que guiará y evaluará el trabajo diario del alumno.

El VHIR ofrece a todos los estudiantes la posibilidad de realizar las prácticas en uno de los laboratorios del Instituto de Investigación. Los estudiantes interesados ​​deben dirigirse por correo electrónico a la persona de contacto que aparece en cada oferta.

Una vez hayas sido admitido para realizar prácticas en un grupo de investigación o una empresa externa, deberás dirigirte a la Secretaría Académica del VHIR para firmar el convenio de colaboración y hacer todos los trámites necesarios para las prácticas.

Centros y entidades colaboradoras

Las ofertas de prácticas disponibles actualmente son:

Diagnostic Immunology

Tutor: Dr. Clara Franco Jarava| Data d’obertura: 21/09/2017 | Centre en pràctiques: Hospital Vall d'hebron
1

Research Line: Immunogenetics

HLA association to adverse reactions to Benznidazol in Chagas disease. The tasks to be performed by the student selected are the following:
(1) Coordination, data collection and samples management (200 national patients and 240 patients from 4 different countries)
(2) DNA extraction and quantification from blood and dry spots
(3) HLA typification through SSO-PCR (Luminex technology) (4) Statistical analysis of the results.

Recent publications:

-Salvador F, Sulleiro E, Sánchez-Montalvá A, Martínez-Gallo M, Carrillo E, Molina I. Impact of Helminth Infection on the Clinical and Microbiological Presentation of Chagas Diseases in Chronically Infected Patients. PLoS Negl Trop Dis. 2016 Apr 26;10(4):.

-Salvador F, Sánchez-Montalvá A, Martínez-Gallo M, Sala-Cunill A, Viñas L, García-Prat M, Aparicio G, Sao Avilés A, Artaza MÁ, Ferrer B, Molina I. Evaluation of cytokine profile and HLA association in benznidazole related cutaneous reactions in patients with Chagas disease. Clin Infect Dis. 2015 Dec 1;61(11):1688-94.

-Franco-Jarava C, Álvarez de la Campa E,Solanich X, Morandeira-Rego F, Mas V, Garcia-Prat M, de la Cruz X, Martín-Nalda A, Soler-Palacín P, Hernández-González M, Colobran Oriol, R. Early versus late diagnosis of complement Factor I deficiency: Clinical consequences illustrated in two families with novel homozygous CFI mutations. Journal of Clinical Immunology.2017. Accepted manuscript. DOI: 10.1007/s10875-017-0447-x

-Franco-Jarava C, Comas D, Orren A, Hernández-González M, Colobran R.
Complement factor 5 (C5) p.A252T mutation is prevalent in, but not restricted to, sub-Saharan Africa: implications for the susceptibility to meningococcal disease. Clin Exp Immunol. 2017 Aug;189(2):226-231.

-Franco-Jarava C, Colobran R, Mestre-Torres J, Vargas V, Pujol-Borrell R, Hernández-González M. Clinical laboratory standard capillary protein electrophoresis alerted of a low C3 state and lead to the identification of a Factor I deficiency due to a novel homozygous mutation. Immunol Lett. 2016 Jun;174:19-22. doi: 10.1016/j.imlet.2016.04.011. Epub 2016 Apr 14.

-Colobran R, Franco-Jarava C, Martín-Nalda A, Baena N, Gabau E, Padilla N, de la Cruz X, Pujol-Borrell R, Comas D, Soler-Palacín P, Hernández-González M. Novel Mutations Causing C5 Deficiency in Three North-African Families. J Clin Immunol. 2016 May;36(4):388-96.

From September 2017 to September 2018

cfranco@vhebron.net

Diagnostic Immunology

Tutor: Dr. Manuel Hernandez González and Dr. Romina Dieli Crimi| Data d’obertura: 21/09/2017 | Centre en pràctiques: Hospital Vall d'Hebron
1

Research line: Complement Pathology

The objective is to better understand the role of partial complement deficiencies and antibodies to complemented complexes in the pathogenesis of infections and kidney diseases.

Complement functional assays, ELISA, genetic analysis and sequencing experiments are planned

Recent publications:

-Franco-Jarava C, Álvarez de la Campa E,Solanich X, Morandeira-Rego F, Mas V, Garcia-Prat M, de la Cruz X, Martín-Nalda A, Soler-Palacín P, Hernández-González M, Colobran Oriol, R. Early versus late diagnosis of complement Factor I deficiency: Clinical consequences illustrated in two families with novel homozygous CFI mutations. Journal of Clinical Immunology.2017. Accepted manuscript. DOI: 10.1007/s10875-017-0447-x

-Franco-Jarava C, Comas D, Orren A, Hernández-González M, Colobran R.
Complement factor 5 (C5) p.A252T mutation is prevalent in, but not restricted to, sub-Saharan Africa: implications for the susceptibility to meningococcal disease. Clin Exp Immunol. 2017 Aug;189(2):226-231.

-Franco-Jarava C, Colobran R, Mestre-Torres J, Vargas V, Pujol-Borrell R, Hernández-González M. Clinical laboratory standard capillary protein electrophoresis alerted of a low C3 state and lead to the identification of a Factor I deficiency due to a novel homozygous mutation. Immunol Lett. 2016 Jun;174:19-22. doi: 10.1016/j.imlet.2016.04.011. Epub 2016 Apr 14.

-Colobran R, Franco-Jarava C, Martín-Nalda A, Baena N, Gabau E, Padilla N, de la Cruz X, Pujol-Borrell R, Comas D, Soler-Palacín P, Hernández-González M. Novel Mutations Causing C5 Deficiency in Three North-African Families. J Clin Immunol. 2016 May;36(4):388-96.

From September 2017 to September 2018

aparada@vhebron.net

Diagnostic Immunology

Tutor: Dr. Ricardo Pujol Borrell| Data d’obertura: 21/09/2017 | Centre en pràctiques: Hospital Vall d'Hebron
1

Research line: Thyroid autoimmunity

Project: Role of thyrotropin receptor in thymocytes in early stages of graves disease and thyroiditis mechanisms

The objective is to better understand the role of TSHR in the pathogenesis of autoimmune thyroid disease. Exploratory experiments on the role of negative checkpoints receptors in thyroid autoimmunity are planned.
Transcriptomic, tissue culture, flow cytometry and immunohistochemistry experiments are contemplated.

Recent Publications:

-Colobran R, Giménez-Barcons M, Marín-Sánchez A, Porta-Pardo E, Pujol-BorrellR. AIRE genetic variants and predisposition to polygenic autoimmune disease: The case of Graves' disease and a systematic literature review. Hum Immunol. 2016 A;77(8):643-651.

-Pujol-Borrell R, Giménez-Barcons M, Marín-Sánchez A, Colobran R. Genetics of Graves' Disease: Special Focus on the Role of TSHR Gene. Horm Metab Res. 2015 Sep;47(10):753-66.

-Alvarez I, Collado JA, Colobran R, Carrascal M, Ciudad MT, Canals F, James EA, Kwok WW, Gärtner M, Kyewski B, Pujol-Borrell R, Jaraquemada D. Central T cell tolerance: Identification of tissue-restricted autoantigens in the thymus HLA-DR peptidome. J Autoimmun. 2015 Jun;60:12-9

-Giménez-Barcons M, Colobran R, Gómez-Pau A, Marín-Sánchez A, Casteràs A, Obiols G, Abella R, Fernández-Doblas J, Tonacchera M, Lucas-Martín A, Pujol-Borrell R. Graves' disease TSHR-stimulating antibodies (TSAbs) induce the activation of immature thymocytes: a clue to the riddle of TSAbs generation? J Immunol. 2015 May 1;194(9):4199-206.

From September 2017 to September 2018

aparada@vhebron.net

Growth Factors Group

Tutor: Dr. Joaquín Arribas and Dr. Verónica Rodilla| Data d’obertura: 19/09/2017 | Centre en pràctiques: VHIR
1

Our group has been focused in basic research in breast cancer biology with a clear translational focus. Our lab is interested on targeting specifically senescent cells and studying the consequences of eliminating them at different tumorigenic stages. Senescence is a biological process, which involved cell cycle arrest and it is closely linked to ageing The role of this process is particularly controversial in tumors, since the secretory phenotype associated to these cells can activate the stroma surrounding the primary tumor, promoting metastasis; and on the other hand, in initial stages of tumorogenesis, these cells are able to recruit the immune system allowing the clearing of the tumor cells, suggesting a tumor suppressor activity. We have developed a new set of transgenic mice which will allow us to elucidate the role of these cells during the tumorogenesis.

Between September 2017 and September 2018 (at least 6 months)

Candidates must have an excellent academic record to be eligible for MECD, ISCIII, AGAUR or similar funded fellowships, highly motivated and hard-workers. Candidates with previous research experience will be preferred but not mandatory. Applicants should send a CV and their academic record.

vrodilla@vhio.net or cbernado@vhio.net

Diagnostic Immunology

Tutor: Dr. Mónica Martínez Gallo and Dr. Joan Sayos| Data d’obertura: 19/09/2017 | Centre en pràctiques: VHIR
1

Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening syndrome characterized by persistent macrophages/lymphocytes activation and a multisystem hyperinflammatory syndrome. HLH can be classified into familial or primary (FHL) and acquired or secondary. FHL is caused by homozygous mutations in genes encoding proteins of the cytotoxic pathway. Patients developing a clinical picture of HLH but carrying a single mutated allele, hypomorphic variants or defects in two different HLH-related genes have been recently reported. Monoallelic mutations in HLH-related genes in patients with HLH is a challenge in the diagnosis, and our objective is study the functional and molecular impact of monoallelic mutations involved in Hemophagocytic lymphohistiocytosis in order to determine whether they act as dominant negatives.

From September 2017 to September 2018

Highly motivated students
Good academic marks

mmartinez@vhebron.net

Diagnostic Immunology

Tutor: Dr. Roger Colobran and Dr. Mónica Martínez-Gallo| Data d’obertura: 19/09/2017 | Centre en pràctiques: VHIR
1

Genetic evaluation with massive sequencing tools in a large cohort of patients with common variable immunodeficiency (CVID) under immunoglobulin replacement therapy in Catalonia (the GAMMACAT cohort). A pilot study. The tasks to be performed by the student selected are the following:
(1) Review of the clinical and laboratory criteria to confirm CVID diagnosis
(2) Coordination, data collection and samples management
(3) Collection of clinical samples of those confirmed cases in the initial genetic study.

From September 2017 to September 2018

Physician profile
Highly motivated students
Good academic marks

mmartinez@vhebron.net

Cell cycle and Cancer – Biomedical Group in Gynaecology

Tutor: Dra. Anna Santamaria| Data d’obertura: 07/09/2017 | Centre en pràctiques: VHIR
1

We are a young, newly formed research group at the Vall Hebron Research Institute (VHIR), within the group of Biomedical Research in Gynecology. The primary interest of the group is to understand the mechanisms that give rise to tumorigenesis, with the main focus in ovarian cancer. From a broad perspective, we study mechanisms contributing to aneuploidy with the aim of being able to translate the potential knowledge that we will acquire into the clinics. We are interested in the role of key mitotic kinases and other cell cycle regulators focusing on cancer progression and resistance to chemotherapy, but making it also extensible to other topics such as the role of miRNAs in ovarian cancer progression or genes involved in tumor dissemination. We also cover a transversal line studying the role of mitotic kinases and downstream effectors in Prostate cancer.

between Oct 2017 and Sept 2018

We seek for a motivated student. The ideal candidate should be an organized person, happy to work as part of a team and with good communication skills and a positive attitude. Fluid English and previous experience will be advantageous.

anna.santamaria@vhir.org

Growth Factors Group

Tutor: Dr. Joaquín Arribas and Dr. Verónica Rodilla| Data d’obertura: 21/07/2017 | Centre en pràctiques: VHIO
1

Our group has been focused in basic research in breast cancer biology with a clear translational focus. Our lab is interested on targeting specifically senescent cells and studying the consequences of eliminating them at different tumorigenic stages. Senescence is a biological process, which involved cell cycle arrest and it is closely linked to ageing The role of this process is particularly controversial in tumors, since the secretory phenotype associated to these cells can activate the stroma surrounding the primary tumor, promoting metastasis; and on the other hand, in initial stages of tumorogenesis, these cells are able to recruit the immune system allowing the clearing of the tumor cells, suggesting a tumor suppressor activity. We have developed a new set of transgenic mice which will allow us to elucidate the role of these cells during the tumorogenesis.

between Sept 2017 and Sept 2018 (at least 6 months)

Candidates must have an excellent academic record, minimum grade point average 2.5/4, and be eligible for MECD, ISCIII, AGAUR or similar funded fellowships.
Candidates with previous research experience will be preferred but not mandatory.
Applicants should send a CV and their academic record.

vrodilla@vhio.net and cbernado@vhio.net

Translational Mucosal Immunology Group

Tutor: Dr. Maria Vicario| Data d’obertura: 19/07/2017 | Centre en pràctiques: VHIR
1

Study of the role of specific immune cells (mast cells, eosinophils and B cells) as major players in the control of mucosal barrier mechanisms in health and disease. Our approach to translational research in digestive diseases involves the development of preclinical and clinical studies aimed at identifying biomarkers of gastrointestinal dysfunction and gut health.

October 2017- July 2018

Highly motivated students
Good academic marks

maria.vicario@vhir.org

Cell Signalling and Apoptosis Group

Tutor: Dr. Joan X Comella and Dr. Maria J Perez| Data d’obertura: 19/07/2017 | Centre en pràctiques: VHIR
2

- Role of FAIM-L in neurodegeneration. We are investigating the role of death receptor antagonists, and particularly FAIM-L, in the neuroinflammatory process associated to neurodegenerative diseases, such as Alzheimer's disease. We aim to disclose its role in the shift of the glial response from neuroprotective to neurotoxic, and its interaction with the pleiotropic cytokine TNF. Particular interest is focused on some FAIM-L interacting proteins such as Siva-1.
- Glial-neuron communication through extracellular vesicles has been suggested to play a role in the spreading of AD. We aim at characterize the material that is transferred between neurons and astrocytes to identify cell signaling pathways that contribute to neuronal dysfunction.

Sept 2017-Sept 2018 (flexible dates)

1. Highly motivated student, with good academic records.
2. Good level of English.
3. Ability to work in a multidisciplinary team.

maria.perez@vhir.org

Neuromuscular and mitochondrial diseases

Tutor: Dr. Ramon Martí and other Principal Investigator of the group.| Data d’obertura: 10/07/2017 | Centre en pràctiques: VHIR
3

-Defects of mitochondrial DNA replication
-Defects of mitochondrial translation
-Gene therapy
-Mcardle disease

between Sept 2017 and Sept 2018

For most projects (but not all of them), the lab work implies work with mice

ramon.marti@vhir.org

Group of Biomedical Research in Urology

Tutor: Dr. Rosanna Paciucci| Data d’obertura: 23/06/2017 | Centre en pràctiques: VHIR
1 or 2

A major challenge of metastatic prostate cancer (PCa) is the very frequent acquisition of resistance to the androgen deprivation therapy (ADT), currently the most successful treatment for metastatic PCa. Subsequent chemotherapy of the castration-resistant cancer (CRPC) only shortly extends patient survival. The mechanisms involved in the establishment of CRPC are still unclear. The identification of biomarkers and factors responsible for the development of the CRPC disease are urgently needed to improve survival. Selected cell populations within tumors (cancer stem cells, CSC) have been demonstrated responsible for the generation of metastasis and recurrence. In CRPC clear evidence for a causal role of CSC in the resistance is lacking. Students will participate in on-going experiments aimed to select and characterize CSCs from cell models sensitive and resistant to treatments.

from October 2017 to July/September 2018 (flexible)

Average grade over 2.5
Intermediate or high level of English is desirable
Knowledge or previous experience in molecular biology and cell culture will be also considered

Interested candidates, please send CV to Rosanna Paciucci (rosanna.paciucci@vhir.org).

Translational Molecular Pathology Research Group

Tutor: Dr. Santiago Ramón y Cajal Agüeras / Dr. Stefan Hümmer| Data d’obertura: 20/06/2017 | Centre en pràctiques: VHIR
3

The main objective of our research group is to deepen our knowledge on the molecular mechanisms underlying tumour pathology with a focus on the identification of new diagnostic, prognostic and therapeutic targets. We have chosen to focus on the mechanisms underlying female breast cancer, the most diagnosed malignant neoplasm and the second leading cause of cancer-related deaths. Our current research focuses on two main aspects: 1) the relation of cellular stress to cancer progression and resistance to chemotherapeutic treatments and 2) the intra tumour heterogeneity with a focus on the clonal cooperation within a tumour.

Sept 2017 to July/Sept 2018

We are looking for highly motivated candidates, interested in the field of oncology and aiming at pursuing a professional career in the field of Biomedicine, Molecular Biology or other related fields. Candidates must hold a B.S. in Biomedicine or a related field and average score should be at least 2 (scale 1-4). Previous experience in molecular and cell biology techniques, work with animal models as well as good English skills will be positively evaluated.

Please send a cover letter together with a complete CV and the records of the B.S. to sramon@vhebron.net or to stefan.hummer@vhir.org

Advanced Chronic Liver Disease

Tutor: Dr. María Martell and Dr. Salvador Augustin| Data d’obertura: 16/06/2017 | Centre en pràctiques: VHIR
2

1. Nanoparticles of simvastatin as new therapeutic tool for chronic liver disease.
2. Gut microbiota and metagenomic/metabolomic signatures of hepatic endothelial dysfunction in non-alcoholic fatty liver disease

January 2018 to September 2018

Above all, we are looking for highly motivated students and if possible, with good level of english and good academic grades.

maria.martell@vhir.org

Neurovascular research lab

Tutor: Dr. Joan Montaner and Dr. Alejandro Bustamante| Data d’obertura: 16/06/2017 | Centre en pràctiques: VHIR
1

Stroke and brain damage biomarkers. The work will consists of exploring the results of exploring a big antibody library in several stroke patients through statistics and bioinformatics, to select the best candidates and explore them in human samples.

October 2017 to July 2018

Interest in both basic and clinical research

alejandro.bustamante@vhir.org or natalia.corbeto@vhir.org

Experimental Hematology

Tutor: Dr. Marta Crespo| Data d’obertura: 13/06/2017 | Centre en pràctiques: VHIO
2

Mechanisms of pathogenesis and progression in lymphoid malignancies

Sept 2017-Sept 2018 (to be specifically discussed)

- Advanced English
- Priority will be given to candidates with grades superior to 2 (0 to 4 scale)

macrespo@vhebron.net

Group of Biomedical Research in Gynecology – Endometrial Cancer Translational Research

Tutor: Dr. Antonio Gil and Dr. Eva Colás| Data d’obertura: 13/06/2017 | Centre en pràctiques: VHIR
2

Our research follows a “two-way translational approach”: from patient to laboratory and from laboratory to patient to find solutions for unmet clinical issues on endometrial cancer. To do that, we combine molecular, cellular, translational and clinical research, in three main research lines; those are implementation of molecular diagnostics in endometrial cancer, controlling tumor dissemination and recurrence, and developing individualized treatment for endometrial cancer patients .

Oct 2017 to Jul or Sept 2018 (to be agreed with the student according to his/her schedule and the project’s needs)

We are seeking for a motivated student that wants to develop his/her master thesis in a translational research project. Good English skills, certificate in animal manipulation, and marks over 8/10 in BSc will be highly appreciated.

eva.colas@vhir.org

Pediatric Neurology Research Group

Tutor: Dr. Alfons Macaya and Dr. Francina Munell| Data d’obertura: 13/06/2017 | Centre en pràctiques: VHIR
2

1. Neurogenetics of paroxysmal neurological disorders (neuronal channelopathies).
2. Novel therapeutic strategies in Muscular Dystrophies

Sept 2017 to Sept 2018

Specific interest in the field

anna.marce@vhir.org or silvia.ferrer@vhir.org

Translational Molecular Pathology – Gap Junction Lab

Tutor: Dr. Trond Aasen| Data d’obertura: 13/06/2017 | Centre en pràctiques: VHIR
2

We are a young team interested in direct cell-to-cell communication via gap junctions in diseases and in particular cancer. See our review: “Gap junctions and cancer: communicating for 50 years. Nat Rev Cancer. 2016 Dec;16(12):775-788.PMID: 27782134”.
We study the role and regulation of the transmembrane channel proteins — the connexins and the pannexins — promising prognostic markers and therapeutic targets. Techniques used in the lab includes cell communication assays, PCR cloning, cell culture (overexpression and RNAi-knockdown using virus), CRISPR-technology, drug-response studies (cell viability, proliferation and apoptosis assays), protein analysis (Immunohistochemistry, western blot) etc.

We value the contribution of each Master student highly, and will give them plenty of freedom to develop their own project, with full support from the rest of the team members. Possible student projects will be tailored to the latest results and the experience of the student, examples:

1) The translational regulation of connexins: The simple dogma that one mRNA produces one protein is changing. We have shown that truncated versions of Cx43 can be generated by direct internal RNA translation. We are elucidating the mechanism and functional consequences. Future aims include: a) Analysing the role of the 3’UTR and analysis of its influence on Cx43 in normal cells and cancer cells. b) Analysis of internal translation in other connexins. Collaboration with other laboratories in BCN possible.

2) Role of truncated Cx43 isoforms in cancer: We have shown truncated isoforms of Cx43 locate to the nucleus and mitochondria and are trying to elucidate the functional consequences. Key approaches include: a) High fidelity CRISPR technology to modulate Cx43 expression in cancer cell lines (including in mouse models). b) Generation of chimeric constructs specifically directing the trafficking of Cx43 to the mitochondria or nucleus. These approaches will be followed by functional analysis of malignant properties (cell migration, apoptosis, resistance to chemotherapeutics etc.)

3) Additional projects exist and can be discussed informally with each student.

The master student will be running their own personal project, and will be directly involved in the development of the experimental procedures. We will provide daily interaction, training, feedback, discussions and will be eager to search for funding for the possibility for a PhD position

Sept 2017 - Sept 2018 (Flexible dates)

- Highly-motivated student with a desire to do a PhD
- High level of English
- Ideally high marks in undergraduate studies (>2 in a scale of 1 to 4).
- Experience working in a research laboratory with techniques of cell and molecular biology.

trond.aasen@vhir.org (attachment of CV, support letters and/or previous mini-projects, will be highly appreciated if available)

Laboratory of Metabolism and Obesity/Group of Diabetes and Metabolism

Tutor: Dr. Josep A Villena| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
2

The main research lines of our laboratory focus at understanding the molecular mechanisms that control energy homeostasis in the organism and how alterations in such mechanisms are linked to the development of metabolic diseases, including obesity, type 2 diabetes or metabolic syndrome.

Our lab offers two positions to carry the experimental work of the VHIR’s Master in Translational Biomedical Research working on the project entitled “Molecular basis of calorie restriction: role of sirtuins and nuclear receptors in mitochondrial biogenesis and glucose homeostasis”.

For decades, it has been appreciated that calorie restriction is the most robust intervention known to extend lifespan in a wide variety of organisms, from yeast to mammals. It also prevents the development of numerous pathologies, including metabolic disorders (i.e. diabetes), neurodegenerative diseases and cancer. Still the exact mechanisms involved in the beneficial effects of calorie restriction remain unknown. In this project, we first aim at uncovering the different pathways and cellular processes regulated by calorie restriction and calorie-restriction mimetics (i.e. resveratrol, SIRT1) by performing microarray-based gene expression profiling studies in adipose tissues of different mouse models. Moreover, we will use tissue-specific knockout mice for PGC-1α, PGC-1β and other regulators of mitochondrial biogenesis (ERRs, MTERFs) in order to study their contribution to the effects elicited by calorie restriction on energy metabolism, as well as their impact on glucose homeostasis and healthy aging. A second research line will focus on identifying new molecules secreted by adipocytes that could be involved in the adipose-pancreas crosstalk. Our research efforts are directed to identify new potential therapeutic targets aimed at improving metabolic and degenerative diseases.

From September 2017 to July/September 2018

We are looking for highly motivated candidates, interested in pursuing a professional career in the field of translational research by joining in the future a PhD program in Biomedicine or similar. Candidates must hold a B.S. in Biology, Biomedicine, Biochemistry or similar. A strong background in molecular and cellular biology, as well as good English speaking and writing skills will be positively valued.

Interested candidates must a letter of intention, CV and academic records to josep.villena@vhir.org

Translational Research in Paediatric and Adolescent Cancer – Paediatric Sarcoma Laboratory

Tutor: Dr. Josep Roma| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
2

The research group is focused on finding new molecular therapeutic targets based on knowledge of the biology of paediatric tumours. The group has identified and continues to identify new molecular targets in soft tissue sarcomas (Notch, Hedgehog, miRNAs, pro-metastatic proteins, etc). The work of the group in recent years has permitted the identification of new potential targets that have recently entered the phase of drug development. In this stage, we have established solid collaborations with the biotechnological industry in order to develop novel small molecules able to inhibit pro-oncogenic processes such as invasion and proliferation with the aim of providing patients with alternative and more specific biological evidence-based therapies. We are convinced that well-directed research not only has to spawn scientific publications but should also lead to the development of new therapeutic compounds that may benefit patients and permit the launch of new biotechnological products on the market.

Between Sept 2017 to Sept 2018. Between this standard period, we can adapt to student’s requirements. We sincerely believe that the contribution of the master students is very important for the group, and therefore, we take care of them and give direct responsibilities of managing a part of a project to the candidate/s.

Good registers (>8)
Please, contact immediately since the selection will be made as soon as possible.

josep.roma@vhir.org

Liver Diseases Research Group

Tutor: Dr. Francisco Rodríguez-Frías| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
1

Quasispecie studies in Hepatitis B and D: analisys by next generation sequencing and in vitro studies.
Our poject consist on searching for conserved motives in X region of HBV genome by NGS technology:
- at nucleotide level as possible targets for gene therapies of HBV chronic infection
- at aminoacid level for prediction or essential domains of X protein

October 2017 to September 2018

No specific requirements

frarodri@vhebron.net

Neurodegenerative Diseases Research Group

Tutor: Dr. Marta Martínez Vicente| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
1

Pathogenic role of autophagic/lysosomal dysfunction in Parkinson's disease

January 2018-Sept 2018

- Experience working in a research laboratory with techniques of cell and molecular biology.
- High marks in undergraduate studies (>2 in a scale of 1 to 4) is preferable.
- High level of English

marta.martinez@vhir.org

Immune Regulation and Immunotherapy Group

Tutor: Dr. Joan Sayós| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
1

In this project we propose two major objectives. First determine whether inhibitory receptors (CD300a, CD300f and LAIR1) expressed by cells of the immune system may constitute therapeutic targets for the treatment of cancer. Our second aim is to demonstrate that the therapeutic effects of blocking the function of these receptors can be complementary and/or synergistic, opening the door to a combination therapy for the treatment of cancer by modulating the function of different immune cells at the same time. First, we will analyze the role of receptor CD300f in the formation and suppressive function of the MDSC and how the receptors LAIR1 and CD300a could modulate the antitumor capacity of NK and T cells. Next we will analyze the therapeutic efficiency of a combined treatment able to decrease the suppressive capability of MDSC and to enhance the activity of NK and T effector cells. A novelty of this project is the use of soluble forms of the receptors as therapeutic tools able to block the function of the same receptors expressed on cells of the immune system. We will use commercial soluble recombinant proteins as well as a chimeric protein procedure that makes that the tumor cells could produce the soluble proteins by themselves. This system is based on the TIM1 molecule, which releases its extracellular domain by protease activity in renal tumors. To develop these studies, we will use “ex vivo” primary human cell cultures of NK cells, lymphocytes T CD8+ and MDSC, as well as mouse syngeneic tumor models. The experimental models that we propose to develop in this project will not only help to validate the potential therapeutic role of the specific receptors object of this study, but it will allow us to establish a platform to systematically evaluate the anti-tumor potential of new proteins in the future.

Sept 2017- July 2018

No specific requirements.

joan.sayos@vhir.org

Laboratory of Cardiovascular Diseases

Tutor: Dr. Javier Inserte| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
1

Iron deficiency is one of the most prevalent nutritional deficiencies in the world population. However, it is unknown whether this deficiency modifies myocardial tolerance to ischemia or the progression to post-infarction adverse ventricular remodeling, and information about the immediate effects of iron replacement in patients with acute heart failure (AHF) is scarce. The student will participate in a project that addresses the effect of iron deficiency induced by diet on the tolerance to myocardial ischemia and the occurrence of post-infarction adverse ventricular remodeling in an in vivo mouse model with transient coronary occlusion. The involvement of oxidative/nitrosative stress and eNOS/sGC/PKG pathway inhibition will be analyzed. In the same model, reversion of iron deficiency effects will be tested by iron reposition and activators of the guanilyl cyclase.

Sept 2017 - Sept 2018

Highly motivated students
High marks is preferable(>2 in a scale of 1 to 4)

javier.inserte@vhir.org

Functional Validation & Preclinical Research (FVPR) / Drug Deliver & Targeting

Tutor: Dr. Ibane Abasolo| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
3

As part of Drug Delivery and Targeting Group (http://www.cibbim.eu/services/drug-delivery-and-targeting/) we work on the development, testing and validation of new therapeutic strategies based on nanotechnology, with which to provide solutions to clinical needs not covered in the fields of oncology and some rare diseases. In short, it is about generating new scientific / biomedical knowledge that can be translated efficiently into clinical practice. In this regard, we offer the master students the possibility of joining one of these 3 projects with clear translational possiblilities:

1. PENTRI: Personalized nanomedicine for triple negative breast cancer
The PENTRI project is aimed at developing novel nanotechnology based strategies to selectively target cancer stem cells (CSC) in triple negative breast cancer cells. The master student joining this project will be in charge of testing in vitro the anti-CSC activity of bare drug candidates and novel drug conjugates. Previous knowledge in tissue culture and general molecular biology techniques will be positively valued.

2. NoCanTher: Nanomedicine Upscaling for Early Clinical Phases of Multimodal Cancer Therapy
This H2020 project (http://www.nocanther-project.eu/) seeks the development of iron nanoparticles as medical devices for the treatment of locally advanced pancreatic cancer with hyperthermia. The master student joining this project will be in charge of characterizing different batches of nanoparticles in terms of cell internalization, X-ray contrast, tumor permeation and response to magnetic field.

3. Smart4Fabry: Smart multifunctional GLA-nanoformulation for Fabry disease
The Smart4Fabry is a H2020 project (http://cordis.europa.eu/project/rcn/206748_es.html) where our role is to test the efficacy of liposomal systems to deliver a therapeutic protein (alpha-galactosidase enzyme) to the lysosomes of patients affected with Fabry disease. The master student joining this project will be in charge of testing the enzymatic activity, cell internalization, and cellular activity of GLA-loaded liposomes in specific primary cultures. Assays determining the pharmacokinetic profile and the in vivo efficacy of the liposomes are also foreseen.

It is worth noting that master students will be running their own personal project under the supervision of lab personnel and Dr. Abasolo and their will be directly involved in the development of the experimental procedures. Although daily interaction, training and feedback will be provided, we do expect students to be capable of leading their subproject. Great individual and team working abilities, good communication skills and good knowledge of English are required.

Sept 2017 to Sept 2018 (flexible)

1. Highly motivated students
2. Good communication skills and fluent English
3. Degree average score above 1.8 (scale 1 to 4).
Send complete CV and academic records along with the application.

ibane.abasolo@vhir.org

Biomedical Research in Digestive Tract Tumors – CIBBIM Nanomedicine

Tutor: Dr. Diego Arango| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
1

1) Investigation of the molecular mechanisms of colon cancer. Different techniques of cell and molecular biology will be used to study the molecular mechanisms involved in the initiation and progression of colorectal tumors using in vitro cellular systems, animal models and large collections of human primary tumors.

2) Identification and validation of new molecular markers of response to treatment for cancer. Here, high throughput techniques will be used to identify new biomarkers of response to chemotherapeutic treatment for colorectal cancer patients, that will subsequently be validated in large collections of patients with colorectal cancer that received chemotheapy.
PERIOD FOR THE INTERNSHIP : September 2017-september 2018

More info: https://goo.gl/lMYY41

September 2017-September 2018

Experience working in a research laboratory with techniques of cell and molecular biology.
High marks in undergraduate studies (>7.5 on a scale 1 to 10) is preferable.
High level of English

diego.arango@vhir.org

Pulmonology

Tutor: Dr. María Jesús Cruz and Dr. Susana Gómez| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
2

Students will be included in one of the following research lines:

- Asma and Pollution (Dr Cruz)
- Lung Transplant (Dr Gómez)

More information at: http://www.vhir.org/portal1/grup-presentacio2.asp?s=recerca&contentid=187034&idrefer=187035

between Oct 2017 and June 2018

No specific requirements

mj.cruz@vhir.org

Gut Microbiota

Tutor: Dr. Chaysavanh Manichanh| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
1

Meta-omics approaches have been intensively used over the last 15 years to study the composition and functions of the human microbiome (the other Human Genome) in health and disease conditions. The aim of our research is to understand the mechanism in which the gut microbiome is involved in the development and perpetuation of intestinal disorders. Here is an example of the kind of research we are doing: https://www.youtube.com/watch?v=Y21reEQ90g4&list=PL80fezoRY4BDr-oZjQVFikD4M2dtRDS6b
More info: https://sites.google.com/site/manichanhlab/home

No specific requirement

Marks for BSc over 7.5/10
Advanced English
Certificate in animal manipulation would be appreciated

cmanicha@gmail.com

Cardiovascular Diseases Research Group

Tutor: Dr. Antonio Rodríguez Sinovas| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
1

The student will be included in one of the following research lines:

A. Role of Connexin Cx43 in myocardial scar formation, left ventricular remodeling and heart failure.
Background: Connexin 43 (Cx43) is essential for cardiac electrical coupling, but also in acute ischemia-reperfusion injury and cardioprotection. Although Cx43 is involved in wound healing and scar formation in the skin, its role in the chronic phases of myocardial infarction and during left ventricular remodeling is unknown. Previous studies in bone tissue suggested a link between Cx43 and lysyl oxidase (LOX), the first enzyme
catalyzing collagen cross-link formation. Furthermore, genomic analysis have demonstrated upregulation, in hearts from Cx43-deficient mice, of genes coding for some LOX isoforms.
Main objectives: (1) To assess the role played by Cx43 in myocardial scar formation, left ventricular remodeling and heart failure in a Cx43-deficient in vivo mouse model of permanent coronary ligature or transient ischemia followed by reperfusion; (2) to analyze the involvement of LOX in the observed effects, and the link between both proteins; and (3) to study the mechanisms involved in the effects of Cx43 in myocardial scar formation and left ventricular remodeling, including channel-independent effects and activation of cytosolic signaling pathways.
Methodology: Cx43Cre-ER(T)/fl mice, treated with vehicle or 4-hydroxytamoxifen to induce a marked Cx43 reduction, and their corresponding controls (Cx43fl/fl), and TgLOX mice overexpressing LOX, will be submitted to permanent left coronary artery ligature (16-23 days) or to 45 min occlusion followed by reperfusion (16-23 days). Infarct size, myocardial function, and the degree of collagen organization will be
compared between groups. Mechanisms will be explored in ventricular samples obtained from these hearts, and in fibroblasts isolated from additional hearts.
Expected results: Cx43 may play an important role in the chronic phases of myocardial ischemia-reperfusion injury, modulating scar formation after infarction, left ventricular remodeling and heart failure. These actions would be modified in Cx43-deficient mice, in a LOX-dependent manner.

B. Translational approaches for myocardial protection. Using an in situ heart pig model we assess the cardioprotective effects, and the mechanisms involved, of several new therapeutic strategies that can be later translated to the clinical arena.

Between November 2017 and September 2018

Good english level
Good academic records

antonio.rodriguez.sinovas@vhir.org

Neurovascular Diseases Research Group (Neurorepair)

Tutor: Dr. Anna Rosell| Data d’obertura: 12/06/2017 | Centre en pràctiques: VHIR
1

The Master Student will join a research team studying brain neurorepair and potential therapies to enhance neurovascular remodelling and neurogenesis after cerebral ischemia including cell-therapy, neurorehabilitation and endogenous brain plasticity. Our goal is to study both angiogenesis and neurogenesis in experimental stroke models and in clinical human studies in order to identify how to potentiate them correctly and how to improve brain function and neurorecovery after stroke from a translational perspective.
The Master students will participate at different levels to one clinical study involving stroke patients receiving rehabilitation therapies at our center and to preclinical study in a mouse model of stroke of neurorepair. More info: www.lin-bcn.com and www.vhir.org

November 2017 to July 2018

Basic cellular and molecular biology techniques such as cell culturing, western blot, RT-PCR, etc.

Send your CV and academic records to anna.rosell@vhir.org

Neurovascular Research Lab- Cerebral Amyloid Angiopathy

Tutor: Dra. Mar Hernández Guillamon| Data d’obertura: 13/06/2017 | Centre en pràctiques: VHIR
1

The impaired β-amyloid (Aβ) clearance and a deficient efflux across the blood–brain barrier (BBB) are key points involved in the accumulation of Aβ in Alzheimer’s Disease (AD) and Cerebral Amyloid Angiopathy (CAA). Due to the evident involvement of certain Apolipoproteins, such us determined ApoE isoforms, these lipid-carriers appear to be excellent candidates to mediate the Aβ traffic through the BBB. In the present project, we aim to deeply investigate the association of different variants of apolipoproteins and Aβ, regarding BBB-crossing efficiency and their ability to mobilize Aβ using in vivo and in vitro models of AD/CAA.

Oct 2017 to Sept 2018

Highly-motivated students and interested in neuroscience

mar.hernandez.guillamon@vhir.org